Luminex 100 IS User Manual, Version 2.1 - Manual (Page 149)

x MAP Technology Troubleshooting
PN 89-00002-00-061 Rev. A 9 - 3
Luminex 100 Analyzer
Fails Calibration
Perform the following items in the order they appear. If you find that
your problem is solved before getting too the last item, you do not
need to perform the rest of the items.
• View the calibration trend report. Check for any dramatic
changes in temperature, sheath pressure, or voltages.
• Ensure that the calibration microspheres were properly mixed
before they were dispensed into the wells.
• Check lot numbers and target values entered into the Update
CAL Targets dialog box.
• See if you added the system calibrators to the correct well on the
plate.
• Check for sufficient volume added to the well.
• Complete the following analyzer functions from the Maintenance
tab: Drain, Backflush, and Alcohol Flush.
• Calibrate the system.
• Open a new bottle of calibration microspheres and repeat the
calibration process.
• Replace the XYP instrument Sample Probe with the short
Sample Probe and repeat the suggestions above. If the system
can be verified using the short Sample Probe, you may need to
readjust the Sample Probe height or ensure that the XYP
instrument Sample Probe is not blocked.
Luminex 100 Analyzer
Fails System Controls
• View the system control trend report.
• Ensure that the system control microspheres were properly
mixed before they were dispensed into the wells.
• Check the lot numbers and target values entered into Update
CON Targets dialog box.
• See if you added the system control microspheres to the correct
wells on the plate.
• Check for sufficient volume added to the well.
• Complete the following analyzer functions from the Maintenance
tab: Drain, Backflush, and Alcohol Flush.
• Calibrate and verify the system.
• Open a new bottle of verification microspheres and repeat the
verification process.
• Replace the XYP instrument Sample Probe with the short
sampling probe and repeat the suggestions above. If the system
can be verified using the short Sample Probe, you may need to
readjust the Sample Probe height or ensure that the XYP
instrument Sample Probe is not blocked.