Luminex 100 IS User Manual, Version 2.1 - Manual (Page 165)

x MAP Technology Glossary
PN 89-00002-00-061 Rev. A 11 - 3
laser Light Amplification by Stimulated Emission of Radiation (laser).
This highly purified source of light is an efficient way to excite
fluorochrome electrons.
microparticle A solid substance with a diameter in the micrometer range. Often
used as a synonym for a microsphere.
microspheres Polystyrene spheres with a diameter in the micrometer range. Also
called beads.
multi-analyte Several assays or tests performed simultaneously in the same
reaction container.
multi-batch A set of batches to be processed consecutively.
photobleaching The process in which light absorption converts the fluorochromes
inside the beads into different fluorescent or nonfluorescent
compounds. Photobleaching prevents beads from being properly
classified.
PMT Photomultiplier tube, measures the excitation emission intensity of
the reporter dye bound to the surface of the microspheres.
qualitative Pertaining to calculations that determine the absence or presence of
an analyte.
quantitative Pertaining to calculations that determine the precise numerical
measurement of an analyte.
reporter A molecule (or combination of molecules) with a specific range of
excitation and emission wavelengths that is used to identify or
quantify an analyte. Examples of acceptable reporters for the
Luminex 100 IS are Phycoerythrin and Alexa 532.
reporter channel (RP or RP1) A specific range of wavelengths that includes the emission
wavelength of a designated reporter molecule.
RP1 Refers to the dyes bound to the surface of the microsphere. Also see
reporter channel.
RP See reporter channel.
sample The mixture of assay components (microspheres, reporter, patient
diluent) that are analyzed.