Multichannel Systems MC_Rack Manual - Manual (Page 180)

MC_Rack Manual
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5.4.2 Waveform Shape and QT Interval
For drug screenings, the analysis of the waveform, especially of the field potential duration,
is of main interest. The field potential duration corresponds to the action potential duration,
that is, the QT interval in an electrocardiogram. It is generally measured from minimum of the
Na
+ peak to the maximum / minimum of the IKr current peak.
Matlab based Cardiomyocyte Toolbox (CMC Tools)
This toolbox programmed by Prof. Dr. Ulrich Egert (www.brainworks.uni-freiburg.de) from
the University of Freiburg, Germany, allows an automated detection of the Na
+ peak to the
maximum / minimum of the IKr current peak, and thus automates the measurement of the
QT interval. Also, the RR interval, and the signal frequency can be analyzed.
The CMC Tools are based on Matlab, and are commercially available under licence of the
University Freiburg. Please contact Prof. Dr Ulrich Egert for details.
Mini Analysis Program (Synaptosoft Inc.)
The commercially available program Mini Analysis Program analyzes signal events and time stamps,
for example, the automated detection of the Na + peak and the extraction of the inter-spike-
interval, providing information on the signal frequency and on arrhythmies, or the extraction
of the QT interval.
You can convert the recorded MC_Rack file into the ABF format and then load it directly into
Mini Analysis Program.
5.5 Extracting Parameters
5.5.1 Exporting Parameter Streams to Excel
You can use the ASCII export features of the MC_Rack displays or of MC_DataTool to
export parameter streams to an ASCII file and then import the ASCII file to Microsoft Excel.
You can also use the commercially available program DataAccess Pro from Bruxton
(www.bruxton.com) to open .mcd files directly and import data streams into Excel.
5.6 MC_DataTool
5.6.1 About MC_DataTool
MC_DataTool is an add-on program exporting data retrieved with the MC_Rack program. With
MC_DataTool, you can fast and conveniently convert MC_Rack data files (*.mcd files) into other
file formats.
Supported are the following input data streams: Electrode Raw Data, Analog Raw Data,
Filtered Data, Spikes, Parameter, Trigger, Digital Data. Specific traces can be selected and
saved for further processing as an Axon Binary File (*.abf), an ASCII file (*.txt), or a binary file
(*.raw). The Averager input stream can be saved as ASCII file only.