Viewing fraction collection results — the heat map – Bio-Rad ChromLab™ Software User Manual
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| Evaluating Results
248
| NGC Chromatography Systems and ChromLab Software
Viewing Fraction Collection Results — the Heat Map
A configuration of the fraction collector (the rack display or the outlet valve) appears
to the left of the Fractions table. The image displays the configuration of the
selected fraction collector when the run was executed. After peak integration, a
heat map of the different fraction properties appears. If a multi-wavelength UV
detector was used for the run, you can select a trace for the heat map (the default
trace is 280 nm). The color of the heat map is based on the color of the chosen trace
in the chromatogram. Tubes or outlet valve ports that do not contain fractions
appear shaded.
Note:
Because OV1 Port 1 directs flow either to the diverter valve of the
BioFrac fraction collector or to waste, it always appears shaded in the outlet
valve display. When two outlet valves are used to collect fractions, OV1Port 12
also appears shaded.
You can base the heat map on any of the following columns in the Fractions table by
selecting from the Color by dropdown list:
Area
Concentration
Amount
Molarity
280/260 (or 255)