Bio-Rad ChromLab™ Software User Manual
Page 171
Creating a Method from Standard Phases
User Guide
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Serpentine is selected by default. Fractions can also be collected by rows or
columns for microplates. Possible choices appear for the rack you select.
If you have outlet valves in the fluidic scheme, choose a starting valve and port
and modify the fraction size.
8.
Under Flow, specify the default flow rate for the method. You can change the
default flow rate within individual phases if needed.
Select the Control the flow to avoid overpressure checkbox if you want the flow
rate to decrease and the method to continue running in the event that the
system reaches high pressure limits. If you do not select this checkbox the
system pumps will stop, pausing the method.
If you have an air sensor installed and you want to monitor and stop the system
pumps if air is detected in the lines, select the Detect end of buffer with Air
Sensor checkbox.
Note:
If the method pauses due to an end-of-buffer signal, the fluidic lines
must be primed before resuming the method. Because the system pump’s
Purge button is inactive in Method mode, you must reprime the pumps
manually. See
Priming and Purging the System on page
for information