Bio-Rad ChromLab™ Software - Manual (Page 152)

5 | Method Editor
150 | NGC Chromatography Systems and ChromLab Software
 Column switching valve
 Outlet valve
 Sample inlet valves
 Wash solution is delivered through Q1 for the sample loop, pH flow cell,
and column switching valve.
 Sample inlet valves are washed by using the sample pump to pull wash
solution through ports 1–8 on S1 (if only one sample inlet valve is present)
or ports 1–7 on S1 and ports 1–8 on S2 (if two sample inlet valves are
present).
 Ports Q1, Q2, Q3, and Q4 are washed individually with 5 ml.
 Buffer inlet ports A1–7 and B1–7 are not washed.
To clean the flow paths completely
 Clean the manual inject port and pH valve manually.
Ensure that the pH probe is removed from the pH valve and that all column
ports are connected to bypass tubing.
To clean the pH valves
1. Open the System Control window.
2. Select Tools > Calibrate to open the Calibration dialog box.
Note: No calibration is performed in this procedure.
3. Choose pH in the Calibrate dropdown list.
4. Click Start to set the valve flow path.
5. Fill a syringe with 1 M NaOH, connect the syringe to the pH valve Cal In port,
and inject the solution.