Bio-Rad ChromLab™ Software - Manual (Page 192)

6 | Creating a Method
190 | NGC Chromatography Systems and ChromLab Software
Special Considerations
Rules for generating scout sequences can vary depending on the parameter chosen
to scout. This section provides a list of considerations to take into account when
generating a scout sequence.
Generating %B Scout Sequences
 You can scout on two or more steps with the same starting %B values. If
you select two or more steps with different starting values, you must clear
the steps with the different values.
 When a linear gradient is selected for scouting and the value for the next or
previous %B step is the same as the selected gradient, that step is
automatically selected for scouting. Clear that step if it is not required.
Note: If an isocratic step is selected for scouting, no other steps are
automatically selected. You can choose others manually.
 When the starting value for %B is 100, the increments (on Page 3) default
to a negative value.
Generating pH Scout Sequences
 This option is available only when the fluidic scheme includes the buffer
blending valve and buffer blending is selected in Method settings.
 The minimum and maximum pH scouting range is defined by the buffer
selection made in Method settings.
Generating Duration Scout Sequences
 Choosing two or more steps that have different starting values or that have
different units is not supported. Clear the steps with different starting
values or units to proceed.