Bio-Rad BioLogic DuoFlow™ pH Monitor - Manual

1
The BioLogic pH monitor is an in-line electrode and flow cell that provides direct monitoring of pH conditions during
a sample run. It provides real-time monitoring of buffer pH and is designed for flow through pH recordings. The pH
monitor is available as an option to the BioLogic DuoFlow systems and is compatible with the discontinued
BioLogic HR systems. The monitor ships with all Maximizer systems.
The monitor is supplied with a tubing kit that includes installed ¼ -28 fittings. When used with either a DuoFlow or
HR system the pH monitor connects via the Signal Import Module (SIM). When a Maximizer is installed with the
DuoFlow Workstation the monitor connects directly to the system via a BNC port on the Maximizer.
The pH electrode is a sealed Calomel combination electrode consisting of a pH and a reference electrode built into
a single body. The sealed reference design eliminates the need to add electrolyte solutions and minimizes
reference dry out. The pH electrode is fully compatible with buffers (such as Tris) that may not be compatible with
Ag/AgCl containing electrodes. The pH Monitor is designed for use in chromatography applications at flow rates up
to 80 ml/min and a flow cell pressure less than 75 psi.
The flow cell is made of PEEK and has a swept volume of approximately 80ul when the pH electrode is inserted.
These instructions cover the installation, calibration, and maintenance of the pH electrode and flow cell. Be sure to
keep this information with your BioLogic DuoFlow or HR instruction manual for future reference.
Installation Instructions
Figure 1. pH Electrode and Flow Cell
The flow cell and pH electrode should be plumbed downstream of any backpressure device or detector. The pH
Monitor has a maximum pressure rating of 75 psi.
DuoFlow pH Monitor
Catalog Number 760-2040