Bio-Rad ChromLab™ Software - Manual (Page 104)

4 | Performing a Manual Run
102 | NGC Chromatography Systems and ChromLab Software
Cleaning the System
System cleaning consists of rinsing the system to remove buffers, washing with
base to hydrolyze any adsorbed proteinaceous material from the fluidic system, and
rinsing again to remove the base. The system can also be cleaned in method mode.
See Chapter 5, Method Editor for details.
To clean the system manually
1. Take the column offline.
2. Place both pump inlet lines in water, set %B to 50%, and flush the system while
simultaneously switching valves through each position.
3. Place both pump inlet lines in 1 M sodium hydroxide, set %B to 50%, and flush
the system while simultaneously switching valves through each position.
Monitor system cleaning using the single- or multi-wavelength conductivity
monitor. Flush until conductivity monitor reads max conductance and is stable.
4. Place both pump inlet lines in water, set %B to 50%, and flush the system
thoroughly to remove all sodium hydroxide while simultaneously switching
valves through each position. Monitor the system rinsing using the conductivity
monitor. Rinse until conductivity monitor reads zero.
5. (Optional) Place both pump inlet lines in storage solution (for example, 20%
ethanol), set %B to 50%, and flush the system with the solution while
simultaneously switching valves through each position.