Bio-Rad ChromLab™ Software - Manual (Page 143)

Phase Controls and Parameters
User Guide | 141
Injecting Sample Directly with a Sample Pump
When a sample pump is present in the fluidic scheme and the sample is injected
directly onto the column, you have the option to interrupt the injection if air is
detected. If you select this checkbox and air is detected in the line connected to the
air sensor set to detect end of sample, the system stops the sample pump and the
method continues to the next step.
Fluidic Scheme Includes a Sample Inlet Valve
If your fluidic scheme includes at least one sample inlet valve and you choose to
inject the sample directly onto the column, you have the option to perform a
preinjection wash with buffer to flush the sample pump and lines and the loop. If you
select this checkbox, you can choose the port on the sample inlet valve from which
to draw buffer solution. You can define the flow rate and the volume of buffer for the
preinjection wash.
You also have the option to flush the sample pump and related connections after
sample injection. Again, you can choose the port on the sample inlet valve from
which to draw buffer solution and define the flow rate and the volume of the buffer
for the postinjection wash.