Column performance test parameters – Bio-Rad ChromLab™ Software User Manual
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Phase Controls and Parameters
User Guide
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Column Performance Test Parameters
This test combines the three basic phases necessary for a run (equilibration, sample
application, and elution). After equilibrating the column, a non-adsorbing sample,
such as acetone or salt, is injected via the sample loop and eluted under isocratic
conditions. After the run, calculate the column efficiency in terms of height
equivalent to a theoretical plate (HETP) and the peak asymmetry factor (As).
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