Bio-Rad BioLogic Signal Import Module (SIM) User Manual
Page 94

4.1
GENERAL GUIDELINES FOR CREATING YOUR OWN TUBING CONNECTIONS
The DuoFlow system uses three types of tubing. Use the following table to select the appropriate tubing.
The Fittings Kit comes with fittings, tubing, and an F10 Tubing Kit, with the necessary tubing cut and
fitted for each system connection (see Figure 4-1). Complete description of each of these items is
provided with the Fittings kit.
Table 4-1.
Tubing Guidelines
Use
Tubing Dimensions
Tubing Material
Fittings
Vol/cm
Pre-pump*
1/8” OD x 0.062” ID
clear,
PTFE
Super flangeless fittings
20 µl
(Workstation
(3.2 mm OD x 1.6 mm ID)
for 1/8” (3.2 mm) OD tubing
and Aux pumps)
Post-pump**
1/16 OD x 0.02” ID
clear, Tefzel
Super flangeless fittings
2 µl
(1.6 mm OD x 0.5 mm ID)
for 1/16” (1.6 mm) OD tubing
Post-pump**
1/16” OD x 0.020” ID
orange, PEEK
1/4-28 Super flangeless fittings 2 µl
with F10
(1.6 mm OD x 0.5 mm ID)
for 1/16” (1.6 mm) OD tubing
pumphead
Post-pump**
1/16” OD x 0.030” ID
green, PEEK
1/4-28 Super flangeless fittings 4.5 µl
with F40
(1.6 mm OD x 0.76 mm ID)
for 1/16” (1.6 mm) OD tubing
pumphead
* Pre-pump refers to all tubing leading up to pump inlet port. Pumps include Workstation pumps, EP-1 pump, and Econo Gradient Pump. Maximizer uses
pre-pump tubing.
**Post-pump refers to all tubing following the pump outlet port.
When plumbing the system, be sure to keep tubing lengths to a minimum. All fittings should be finger-tight.
Do not over-tighten, as you risk damaging the connection.
Ferrule Installation: 1/8” (3.2 mm) OD and 1/16” (1.6 mm) OD tubing
1.
Cut the tubing with the tubing cutter provided in the Fittings Kit. This will give a flat, clean cut to the
tubing; this is important in making a fitting that does not leak.
Figure 4-2. Making 1/4-28 flat bottom fittings
TUBING
CUT THE
TUBING
NUT
STAINLESS STEEL
COMPRESSION RING
PEEK FERRULE
FITTINGS TIGHTENER
FITTINGS
TIGHTENER
SYSTEM PLUMBING
SYSTEM INSTALLATION AND SETUP
4-2
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