Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 192)

REVERSE FLOW CHROMATOGRAPHY ADVANCED SYSTEM APPLICATIONS
10-5
(Optional) Check the pH Correction
9. From the manual screen press the Buffer Blending "Setup" button and select the desired buffer
system from the Buffer System list. Turn pH correction on and enter the Desired and Observed pH
from step 8 above.
10. Set the pH, Salt composition and flow rate. Press run.
11. After the system has equilibrated collect some effluent and measure the pH.
Figure 10-1. Buffer Blending Setup Dialog for a Single Component Buffer
Figure 10-2. Buffer Blender Setup Dialog for a Multi-Component Buffer
Two point correction (best for gradient experiments)
1. From the manual screen Press the Buffer Blender "Setup" button and select the desired buffer
system. Make sure the pH correction box is not checked.
2. Set the pH and flow rate. Set the salt composition to 0 %B. Press run.
3. After the system has equilibrated collect some effluent.
4. Set the salt composition to the maximum %B that will be used in the experiment. Press run.
5. After the system has equilibrated collect some effluent in a second tube.
Formate/Acetate/Phosphate pH 3.10 to 6.80
Buffer Blender
Buffer System
Buffer System: Formate/Acetate/Phosphate
Sort By: Name pH Range
pH Range (at 25 Celcius)
Molarity at 100%B:
Temperature Compensation Coefficient:
pH Correction Values
Use pH Correction
3.10 to 6.80
Desired pH pH at 0%B pH at %B
Buffer
100
Acetate 4.40 4.62 4.76
Formate 3.29 1.60 3.75
Phosphate 6.71 7.04 7.20
1.0 M
0.0002
Edit Buffer Blender
*Tris (25 mM) pH 7.1 to 9.1
Solution A1: Acetic Acid (40 mM)
Prepare by dissolving: 80 ml of 1M Acetic Acid in water and diluting to 2.0 L.
Solution A2: Sodium Acetate Teihydrate (40 mM)
Prepare by dissolving: 10.9 g of Sodium Acetate Trihydrate (MW 136.1 g/mol) in water and diluting to 2.0 L.
Solution A3: Sodium Chloride (2.0 mM)
Prepare by dissolving: 233.6 g of Sodium Chloride(MW 58.4 g/mol) in water and diluting to 2.0 L.
Cancel
Buffer Blender
Buffer Blender
Buffer System Sort By: Name pH Range
pH Range (at 25 Celcius)
Molarity at 100%B:
Temperature Compensation Coefficient:
Recipe
OK
pH Correction Values
Use pH Correction
Pump Head Selection: F10
Flow Rate Range: 0.20 - 20.0 ml/min Max Pressure: 700
7.10 to 9.10 Desired pH 4.76
Observed at 0%B pH 4.70
Observed at 0%B 100 pH 4.48
1.0 M
-0.0280
psi
Use two point correction