Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 3)

2.9.8 Model 1327 Chart Recorder...........................................................................2-52
2.9.9 Generic Chart Recorders ...............................................................................2-52
2.9.10 Uninterruptible Power Supply.........................................................................2-53
2.9.11 Printers ...........................................................................................................2-53
2.10 Columns and Column Fittings.....................................................................................2-54
2.10.1 Anion Exchange: Q-Strong Anion Exchange ................................................2-54
2.10.2 Cation Exchange: S-Strong Cation Exchange..............................................2-54
2.10.3 Anion Exchange: DEAE Weak Anion Exchange...........................................2-55
2.10.4 Cation Exchange: Carboxy Methyl (CM) Weak Cation Exchange ................2-55
2.10.5 Ceramic Hydroxyapatite (CHT) ......................................................................2-55
2.10.6 Size Exclusion Chromatography (SEC) .........................................................2-56
2.10.7 High Pressure Reversed Phase Columns .....................................................2-56
2.10.8 Hydrophobic Interaction Chromatography (HIC)............................................2-56
2.10.9 Affinity Chromatography.................................................................................2-57
2.10.10 Empty Columns..............................................................................................2-57
2.10.11 Column Fittings ..............................................................................................2-58
SECTION 2. SYSTEM INSTALLATION AND SETUP
Chapter 3.0 System Setup ............................................................................................................3-1
3.1 Controller Cable Connections .....................................................................................3-2
3.2 USB Bitbus Communicator Cable Connections..........................................................3-3
3.3 Workstation Cable Connections..................................................................................3-4
3.3.1 Systems without a Maximizer ........................................................................3-4
3.3.2 Systems with a Maximizer .............................................................................3-5
3.4 System Rack Setup.....................................................................................................3-6
3.5 Mixers..........................................................................................................................3-7
3.6 Detection System Connections ..................................................................................3-9
3.6.1 UV Detector and Conductivity Monitor...........................................................3-9
3.6.2 QuadTec UV/Vis Detector ..............................................................................3-10
3.6.3 pH Monitor......................................................................................................3-12
3.6.4 Non-Bio-Rad Detectors ..................................................................................3-13
3.7 Valve Connections .....................................................................................................3-14
3.8 Fraction Collector Connections ..................................................................................3-15
3.8.1 BioFrac Fraction Collector..............................................................................3-15
3.8.2 Model 2110 Fraction Collector .......................................................................3-15
3.8.3 Model 2128 Fraction Collector .......................................................................3-15
3.9 Pump Connections .....................................................................................................3-16
3.9.1 Model EP-1 Econo Pump...............................................................................3-16
3.9.2 Econo Gradient Pump (EGP).........................................................................3-17
3.10 Model 1327 Chart Recorder Connections ..................................................................3-19
3.11 Completing System Setup...........................................................................................3-19
3.11.1 DuoFlow System Network Connections.........................................................3-19
3.11.2 System Power Up ..........................................................................................3-19
3.11.3 BioLogic Configuration Utility Software..........................................................3-20
Chapter 4.0 System Plumbing ......................................................................................................4-1
4.1 General Guidelines for Creating Your Own Tubing Connections................................4-2
4.2 Plumbing a DuoFlow System......................................................................................4-3
4.3 Priming the System .....................................................................................................4-8
TABLE OF CONTENTS
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