Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 144)

Table 7-5. Linear Gradient
Use the Edit Linear Gradient screen to deliver buffer gradients. The Linear Gradient screen sets the initial
and final composition of the buffers and the period over which the change in composition is to occur. There
is no limit to the number of gradient steps in a protocol. The following selections are available.
• Buffers: Allows you to choose two buffers from which to make a binary gradient. Drop-down menus
display all buffers that are are assigned to the pump inlets or inlet valves in the device set up (see
Chapter 7.2.2).
• % Composition: Allows you to choose initial and final values for the linear binary gradient. Notice that
changing one value in the “Initial” column affects the other “initial” value. The “Final” column behaves
identically.
The Edit Linear Gradient pH View screen appears when you are using a Maximizer in Buffer Blending
mode. This box sets the pH, initial and final salt concentration, and the period over which the change in
composition is to occur. There is no limit to the number of gradient steps in a protocol. The following
selections are available.
• pH: Allows you to enter the pH for the current step. The dialog box on the left-hand side lists the
currently defined buffer system and its pH range as well as the solutions present at each inlet.
• Initial %B and Final %B: Allows you to set the initial and final salt concentration as a percentage of the
maximium allowable concentration for a linear binary gradient.
The following selections are available in both screens.
• Volume: Allows you to choose the duration of this step.
• Flow: Allows you to choose the flow rate of this step.
• OK: Adds the step to the protocol. This is the same as pressing the Enter key on the keyboard.
• Cancel: Does not add the step to the protocol. This is the same as pressing the Esc key.
• Step, Time or Volume: Identifies current step number, and calculates the elapsed time or volume from
all previous steps. This is not user editable.
Linear
Gradient
Edit Linear Gradient
Step 8, Volume = 2.00 ml
Linear Gradient Screen
Linear Gradient Screen
with a Maximizer in
Buffer Blending Mode
Linear Gradient
Buffer A1
OK Cancel
100 A
Buffer B1
Buffers % Composition
Initial
0
100
Final
0
1.0
Volume (ml.)
1.00 B
Flow (ml./min)
Edit Linear Gradient pH View
Step 2, Volume = 1.00 ml
Linear Gradient pH View
OK Cancel
Buffer System: Tris (25 mM)
pH Range = 7.20 to 9.20
Solution A1: Tris.HCI (50 mM)
Solution A2: Tris (50 mM)
Solution B1: Degassed Water
Solution B2: Sodium Chloride (2.00M)
Molarity at 100%B = 1.0 M
1.00
Volume (ml.)
1.00
Flow (ml./min)
7.00
pH
100
Final %B
0
Initial %B
MODES OF OPERATION SYSTEM OPERATION
7-15