Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 137)

Table 7-1. (continued)
Valve Setup Information
7.2.3 Buffer Editor
The Buffer Editor is used to create new Buffer Blending buffer systems from user-supplied information. This
information is used by the Maximizer to determine the amount of acid, base, water and salt required to
produce a buffer solution at a desired pH and salt composition. Each buffer system can include up to three
buffers and each buffer can have up to three pKa's. The Buffer Editor also generates a recipe describing how
to make each Buffer Blending solution. The Buffer Editor requires information about the buffer ( pKa(s),
temperature coefficient(s), charge state, molecular weight and concentration) and the salt (charge state
molecular weight and concentration). This information can also be supplemented with user determined 1 or 2
point pH correction for each buffer in a buffer system. pH corrections are entered from the Buffer Blending
setup dialog.
MODES OF OPERATION SYSTEM OPERATION
7-8
Valve Type Valve Name/ Position
Function Names
AVR9-8 Aux Pump Inlet Named by user
High pressure
valve
Note: Indicate Inlet A Named by user
the location
of the valve
Inlet B Named by user
cable
connection.
When the
Maximizer is
used, inlets A
and B become Fraction Collector
1 Waste
A1, A2, B1, B2. 2 Collect
3-8 Named by
user
User Assigned Named by user
Name
Column Switching Named by user
Notes
Used for auxiliary pump load selection to select
from up to eight samples, buffers, or rinse
solution.
When used before the inlet to Pump A or B, the
valve provides preparative sample loading or
buffer selection. The buffer or sample name
specified for each position will appear in the
Protocol screen’s “Isocratic Flow”, “Linear
Gradient”, and “Change Valve” dialog box. Refer
to Section 4, Advanced System Applications,
Chapters 8 and 9.
Useful for collecting large volume samples.
Collects up to 8 samples. The name specified for
each position will appear in the Protocol screen’s
“Change Valve” dialog box.
When used for purposes other than described
above. The name specified for each position will
appear in the Protocol screen’s “Change Valve”
dialog box. Refer to Chapters 8 and 9.
Allows you to install up to 8 columns and assign
valve numbers and names to each column.
Requires two AVR9-8 valves: one is an inlet valve
and the other functions as an outlet valve.