Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 44)

2.6.3 SV5-4 Buffer Select and Automated Sample Loading Valve
The SV5-4 valve is a low pressure, 5-port, 4-position valve used for automatic buffer selection and sample
loading. The SV5-4 valve may be used for:
• Buffer and/or sample selection when placed before the Workstation pumps or an auxilary load
pump. An SV5-4 valve connected to a Workstation pump inlet enables access to four separate
solutions.
• Fraction collection when placed after the column.
• Purging or rinsing all tubing lines for cleaning purposes.
Figure 2-15. SV5-4 Buffer Select Valve
Use 1/8” (3.2 mm) OD PTFE tubing and 1/4-28 fittings when plumbing the SV5-4 valve to the Workstation
pump inlet. To prevent air from entering the system, unused ports should be closed with 1/4-28 plugs.
When loading samples directly through the Workstation pump, first filter the sample through a 0.45 µm
filter. Flush the valve pump and lines with a sanitizing solution to remove protein residues after the
sample has been loaded; protein residues might otherwise reduce pump piston seal lifetime.
Connect the SV5-4 valve cable to any of the available solenoid valve connectors on the back of the
Workstation (ports 1, 2, or 3). If a Maximizer is in use, connect to ports 4, 5, or 6 before those on the
Workstation.
From the Manual screen you may manually operate each inlet port on the SV5-4. Manual valve control is
useful for the following:
• When priming the tubing and the valves with buffer and or sample prior to starting a method.
• For purging all tubing lines for cleaning purposes.
Prior to programming a method in the Protocol screen, you may name each of the valves positions in the
Setup screen. The name that you apply in the Setup screen will appear in the method Protocol screen when
you program an Isocratic Flow, Change Valve, or Linear Gradient step.
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COMMON
DESCRIPTION OF BIOLOGIC DUOFLOW SYSTEM SYSTEM OVERVIEW
2-31