Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 143)

Table 7-4. (continued)
Load/Inject Sample
The Fill Before Inject and Rinse After Inject tabs are used to automatically fill a sample loop before an
injection step or to rinse the loop after an injection has occurred. The Fill Before Inject tab is active for both
static loop and dynamic loop injections if an auxiliary pump is defined in the device setup. The Rinse After
Inject tab is active only for static loop injections if an auxiliary pump and auxiliary pump inlet valve are
defined in the device setup.
• Enable Fill Before: Activates the Fill Before Inject controls.
• Enable Rinse After: Activates the Rinse After Inject controls.
• Flow Direction: Sets the flow direction for an Econo Gradient Pump. For an EP-1 pump or non
Bio-Rad pump the flow direction is set at the pump.
• Sample: Used to select a sample or wash solution for use in the Load/Inject step. Active only if an
Aux Pump Inlet valve has been defined for the method.
• Flow: Used to enter the auxiliary pump flow rate. Note that for an EP-1 pump or non Bio-Rad pump
the flow rate must be set at the pump itself.
• Volume: Used to set the amount of solution loaded into the loop from the auxiliary pump.
4
5
3.0
7.0
Linear Gradient
100%
0%
6
100% --> 55%
0% --> 45%
7
23.0
Isocratic Flow
55% --> 100%
45% --> 0% 25.0 Linear Gradient
8
100%
0%
28.0 Isocratic Flow
Load: Sample
End of Protocol
Static Loop
Load: standard
Volume: 1.0 ml
25mM Tris
25mM Tris + Salt
25mM Tris
25mM Tris + Salt
25mM Tris
25mM Tris + Salt
25mM Tris
25mM Tris + Salt
Enable Fill Before
EGP Flow Direction
Edit Load/Inject Sample
Step 4, Volume = 1.00 ml
Load/Inject Sample Fill Before Inject
OK Cancel
Rinse After Inject
This operation will occur BEFORE injecting sample on column.
Rinse
Sample
Sample 1
Sample 2
Sample 3
Sample 4
1.0
Volume (ml.)
1.00
Flow (ml./min)
Enable Fill Before
EGP Flow Direction
Edit Load/Inject Sample
Step 4, Volume = 1.00 ml
Load/Inject Sample Fill Before Inject
OK Cancel
Rinse After Inject
This operation will occur BEFORE injecting sample on column.
1.0
Volume (ml.)
1.00
Flow (ml./min)
Rinse
Sample
Sample 1
Sample 2
Sample 3
Sample 4
SYSTEM OPERATION
7-14