Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 189)

MULTIPLE COLUMNS ADVANCED SYSTEM APPLICATIONS
10-2
10.2 BUFFER BLENDING WITH THE MAXIMIZER
The BioLogic DuoFlow software provides 26 predefined buffer systems for use in Buffer Blending
experiments (see Table 10-1). These buffer systems cover a wide pH range and include most of the
commonly used chromatography buffers. Each buffer system includes recipe text that describes how to
prepare the required Buffer Blending solutions. In addition, the Buffer Editor can be used to create
user-defined buffer systems. The Buffer Editor is a simple tool used to edit (or modify) predefined buffer
systems or to create new buffer systems. Buffer Blending solutions should always be made from reagent
grade materials and then filtered and degassed before use. Filtering the solutions will increase the life of the
DuoFlow check valves as well as the column. Degassing solutions will help prevent air bubbles from forming
in the DuoFlow pumps and the detector.
Table 10-1
Buffer Blending Buffer Systems
Buffer System pH Range*
1 Acetate (20 mM) 3.7 to 5.7
2 Acetate (50 mM) 3.6 to 5.6
3 Bis-Tris (20 mM) 5.6 to 7.6
4 Bis-Tris (50 mM) 5.5 to 7.5
5 Citrate (20 mM) 2.6 to 6.0
6 Citrate (50 mM) 2.4 to 6.0
7 Formate (20 mM) 3.0 to 4.6
8 Formate (50 mM) 2.6 to 4.6
9 HEPES (20 mM) 6.4 to 8.4
10 HEPES (50 mM) 6.4 to 8.4
11 MES (20 mM) 5.1 to 7.1
12 MES (50 mM) 5.1 to 7.1
13 N-Methylpiperazine (pH 4.2 to 5.5) 4.2 to 5.5
14 Phosphate (20 mM) 5.8 to 7.5
15 Phosphate (50 mM) 5.6 to 7.5
16 Phosphate (100 mM) 5.5 to 7.5
17 Phosphate with Ammonium Sulphate 5.6 to 7.2
18 TAPS (20 mM) 7.4 to 9.4
19 Triethanolamine (20 mM) 6.8 to 8.8
20 Triethanolamine (50 mM) 6.8 to 8.8
21 Tris (25 mM) 7.1 to 9.1
22 Tris (50 mM) 7.1 to 9.1
Broad Range Buffers*
23 Bis-Tris/Tris (4 ºC) 6.2 to 9.4 (4ºC)
24 Bis-Tris/Tris (25 ºC) 5.8 to 8.9
25 N-methylpiperazine/Bis-Tris/Tris (25 ºC) 4.7 to 9.4
26 Formate/Acetate/Phosphate (25 ºC) 3.1 to 6.8
* pH range is at 25 C unless otherwise noted