Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 151)

Table 7-10. (continued)
Fraction Collection
Description: Collection Windows
Collection Windows: This method specifies collection during specified parts of the run (Time or Volume).
Each collection window can have a unique fraction size (ml).
Note: Collection windows programming should be done after all other steps have been written. When using
Collection Windows, the BioFrac and Model 2128 will skip a tube between each window collection. For
example, if the first window ends collection at 20 minutes and the second window starts collection starting at
21 minutes, then the fraction collector will leave an empty tube for the period between minutes 20 and 21.
• Fraction Size, Tubes Required, Number of Racks, Start Tube, End Tube, Start Rack and End Rack:
See description for Collect All on page 7-20.
• Add Mode: Enter the desired values for Start, End, and Frac Size.
a.
Select the Save Window button and the values will appear in line 1.
b. Enter the values for the next window and select Save Window. The values will appear in line 2.
c. When the collection scheme is complete, select Finished Adding, and the Select mode will appear.
• Select Mode: This mode (not shown in illustration above) shows the following two buttons:
Add Window: Inserts a window after the highlighted window.
Delete Window: Deletes that window.
Collect All
Threshold
Collection Windows
Threshold and Collection Windows
Fraction Collection Scheme
Close
Waste
Destination:
Non-peak Parameters:
Tubes
Below Threshold
Above Threshold
A
B 90
Rack:
1
1A
1F
Tube #: Row Col.
Start:
End:
1.00
Fraction Size
ml
0.100
Threshold
1.00
4.00 5.00 0.60
Fraction Size
ml
UV Detector
Threshold Detector BioFrac Rack Type: F1 (12-13 mm tubes)
AU
Start
(ml)
CollectionWindows Fraction Collector Scheme
End
(ml) Frac. Size
(ml)
1
2 7.00 8.00 1.00
1
2 7.00
Start
(ml)
Save Window
Finished Adding
0.0
CollectionWindows Fraction Collector Scheme
8.00
End
(ml)
4.0
1.00
4.00 5.00 0.60
Frac. Size
(ml)
0.60
4.00 5.00 0.60
Start
(ml)
Close
Save Changes
EDIT MODE
CollectionWindows Fraction Collector Scheme
End
(ml) Frac. Size
(ml)
1
2 7.00 8.00 1.00
Delete Window
Cancel Editing
Close
Add Window
SELECT MODE
Delete Window
Close
ADD MODE
Delete Window
MODES OF OPERATION SYSTEM OPERATION
7-22