Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 167)

7.5 POST RUN Screen
The Post Run screen enables peak annotation (“tagging”) and data export. To enter the Post Run screen,
open a run file in the Browser screen, or when a run is finished choose PostRun from the toolbar. Two
chromatograms are viewed: the main chromatogram in the lower large window and the reference
chromatogram in the upper right window. In the upper reference chromatogram, use the vertical scrollbar to
adjust the scale.
Figure 7-11a. Post Run Screen showing a Volume-based Chromatogram
and "Rack and Tube#" fraction numbering
Tags
AU
SA
FullView Print Del Tag Activity
Edit View Utilities Options
Edit
Method
New
Method
New
Run Manual Setup
Values at Cursor
Current Tagging Trace:
QuadTec-3 (214 nm)
Run Time:
Gradient:
QuadTec-1 (280 nm):
QuadTec-2 (260 nm):
QuadTec-3 (214 nm):
QuadTec-4 (405 nm):
Conductivity:
GP pressure:
No Trace:
00:03:38.4
21% Buffer B
0.0345 AU
0.0228 AU
0.4762 AU
0.0021 AU
8.47 mS/cm
375.53 psi
Protocol
wash load 1 2
Run Notes Report PostRun Settings Log
Help File
Browser
50
100% Buffer B
QuadTec-3 (214 nm)
AU
GP pressure
50.0
0.0
-10.0
40.0
30.0
20.0
10.0
Fractions
BioLogic Duo-Flow - - - -
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
Split
1.00ml/min 0 %B
Gradient Pump: F-40 UV Conductivity
438 psi 0.03775 AU 359 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.953 Volt 0.00 pH
A
mS/cm
B
0.00
1
1
A
2 3 4 5 6 7 8 9 10 11 12 13 14
10.00 20.00 5.00 15.00 25.00
2
3
4
0.100
0.075
0.050
0.025
-0.000
Milliliters
Rack Pos:
Tube #:
MODES OF OPERATION SYSTEM OPERATION
7-38