Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 175)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
8-2
Figure 8-1. Multiple Sample Loading with an Auxiliary Load Pump and an AVR9-8 Valve
Writing an Automatic Loop Fill and Rinse Protocol
The protocol may be written as a single protocol containing back-to-back experiments or may be written as a
series of queued runs. The latter option generally produces a simpler protocol that may be copied into a
queue (once for each experiment to be run). The Load/Inject step can then be edited for each method in the
queue so that the appropriate sample is loaded.
Several methods that include the Automatic Loop Fill and Rinse feature have been included in the BioLogic
DuoFlow Method Templates (see Section 6.2 and the online help for more information). This feature may be
added to any protocol using a static loop.
1. In the protocol load/inject step select Static Loop as the injection type.
2. In the Fill Before Inject tab, check the Enable Fill Before box, select the sample, and set the sample
volume and flow rate. Note that if an EP-1 or non-Bio-Rad pump is used, the flow rate must be set
both in this dialog and at the pump. Note that the fill time must be less than the duration of the
preceeding step.
3. If the sample loop and tubing are to be rinsed between sample injections select the Rinse After
Inject tab, check the Enable Rinse After box, select the "Rinse" buffer, and set the rinse volume and
flow rate. Note, if an EP-1 or non-Bio-Rad pump is used the flow rate must be set both in this dialog
and at the pump.
8.2 AUX PUMP DIRECT INJECT
With the addition of an Econo Gradient Pump, EP-1 Econo pump or other compatible pump to the DuoFlow
system, samples can be loaded directly onto low-pressure columns. Up to 7 samples can be loaded
sequentially, if an Aux pump inlet valve (AVR9-8) is connected to the system and defined in the setup. One
of the Aux pump inlet valve ports must be assigned to a rinse solution. Similarly, an SVT3-2 or SV5-4 Aux
1
2
3
4
5
6
9
7
8
RINSE
SAMPLE #1
SAMPLE #2
SAMPLE #3
AVR9-8
AVR7-3
LOOP OVERFILL
TO WASTE
LOAD
LOW
PRESSURE
COLUMN
WORKSTATION
PUMP
WASTE
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7
1
2
3
4
5
AUXILIARY
LOAD
PUMP