Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 187)

MULTIPLE COLUMNS ADVANCED SYSTEM APPLICATIONS
9-6
Writing the Protocol
The powerful queuing feature of the BioLogic software makes writing multi-dimensional chromatography
experiments simple. Multi-dimensional experiment can be written as a series of individual one-dimensional
chromatography methods that are then ran sequentially as part of a queued run. Once written, each one-
dimensional method can be combined with other methods to make the desired experiment. For example, a
single affinity protocol could be could be combined with either a desalting method or size exclusion method
to create one of the two-dimensional experiment shown in Table 1. On the other hand an affinity method
could be combined with both a desalting method and an ion exchange method to create the three-
dimensional experiment shown in Table 1.
When writing a multi-dimensional method, care should be taken to prevent reintroduction of impurities
removed in previous dimensions. Cleaning and equilibration steps should be included at the start of each
dimension to wash any impurities from the tubing and to fill the system with the appropriate buffers.