Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 179)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
8-6
8.4 DYNALOOP SAMPLE INJECTION
The DynaLoop, which is available in 25 ml and 90 ml sizes, is used to load large volumes of sample directly
onto chromatography columns. These loops have a sliding piston and function very much like a syringe. The
sample is inserted into the DynaLoop’s sample end connector. The loading of the sample pushes the
DynaLoop’s sliding seal assembly towards the buffer end connector. Note: While other dynamic loops may
be used, the application discussed in this section applies specifically to the DynaLoop.
System Setup
When plumbed directly to the AVR7-3 inject valve, the DynaLoop functions just like a static sample loop. The
sample end of the DynaLoop should be plumbed to port 3 and the buffer end to port 6 of the Inject valve.
The valve’s operation and sample loading are controlled automatically, simplifying the sample injection
process and insuring precise sample loading and gradient formation.
The DynaLoop may be filled either manually with a syringe or automatically using an auxiliary pump such as
an Econo Gradient Pump (EGP) or EP-1 Econo pump that is controlled by the DuoFlow system.
• When using a syringe to fill a DynaLoop, the syringe should be connected to port 2 of the AVR7-3
valve. However, using a 1/4x28 to female Luer adapter between the syringe and the valve makes
filling the loop much easier.
• When using the auxiliary pump to fill the loop, the pump’s outlet plumbing should be connected
directly to port 2 of the inject valve.
Consult the DynaLoop Instruction Manual or DuoFlow online help for additional information. The figure
below shows how to set up a DynaLoop.
Figure 8-5. Plumbing the DynaLoop for use with an Inject Valve
6
7
1
2 3
4
5
LOOP
OVERFILL
TO WASTE
AUXILIARY
LOAD PUMP
SYRINGE OR
AVR7-3
COLUMN
DYNALOOP
WORKSTATION
PUMP
WASTE