Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 140)

Table 7-3.
Isocratic Flow
The Edit Isocratic Flow dialog is used to define the isocratic step buffer composition. The buffer composition
is determined by the percentage of total buffer delivered through pumps A (%A) and B (%B).
• Buffers: Displays all the available buffers. The names displayed for Buffer A and Buffer B are the
names assigned to the pump inlets or inlet valves in the device setup (see Chapter 7.2.2).
• Composition: Allows you to set the buffer composition for the step (e.g., 75% A and 25% B).
The Edit Isocratic Flow pH View dialog is used to define the isocratic step buffer composition when a
Maximizer is connected to the system and Buffer Blending is turned on. The dialog box on the left lists the
current buffer system, its pH range and the solutions assigned to each Maximizer valve inlet.
• pH: Allows you to enter the pH for the current step.
• %B: Allows you to set the buffer composition for the step as a percentage of the 1x salt
concentration.
The following selections are available in both the Edit Isocratic Flow screen and the Edit Isocratic Flow
pH View screen.
• Volume/Time: Allows you to choose the duration of the step.
• Flow rate (ml/min): Allows you to choose the flow rate of the step.
• OK: Adds the step to the protocol. This is the same as pressing the Enter key on the keyboard.
• Cancel: Cancels all input. This is the same as pressing the Esc key on the keyboard.
• Step, Time or Volume: Identifies current step number, and calculates the elapsed time or volume from
all previous steps. This is not user editable.
Isocratic
Flow
Edit Isocratic Flow
Step 2, Volume = 0.00 ml
Isocratic Flow
Buffer A1
OK Cancel
100 A
Buffer B1
Buffers % Composition
0
1.0
Volume (ml.)
1.00 B
Flow (ml./min)
Edit Isocratic Flow pH View
Step 2, Volume = 1.00 ml
Isocratic Flow pH View
Buffer System: Tris (25 mM)
pH Range = 7.20 to 9.20
Solution A1: Tris.HCI (50 mM)
Solution A2: Tris (50 mM)
Solution B1: Degassed Water
Solution B2: Sodium Chloride (2.00M)
Molarity at 100%B = 1.0 M
OK Cancel
1.0
Volume (ml.)
1.00
Flow (ml./min.)
7.00
pH
0
%B
Isocratic Flow Screen Isocratic Flow Screen with Maximizer in Use
MODES OF OPERATION SYSTEM OPERATION
7-11