Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 168)

Figure 7-11b. Post-Run Screen showing a Time-based Chromatogram
and "Rack and Grid#" fraction numbering
7.5.1 Resizing
The main chromatogram shows the current “Zoom” region. To resize the image (or “zoom in” to a particular
region of the chromatogram), either change the axes scale in the reference chromatogram, or use the
“rubber band” controls on either chromatogram. To use the rubber band controls, click and hold the left
mouse button. Drag it across the chromatogram region. Release the mouse button and the main
chromatogram will be resized. Click on the Full View button in the toolbar to return to normal view.
7.5.2 Chromatogram Information (Values at Cursor)
The top left corner of the PostRun screen contains information about the chromatogram. The Values at
Cursor window in the upper left corner will list up to eight detector traces depending on the devices selected
in the runs device setup. The position of the vertical bar within the main chromatogram dictates the displayed
information. Run time, absorbance (AU), conductivity (mS/cm), gradient progression (%B), and the activity
trace values are displayed for each trace at the position of the vertical bar. To view the vertical bar, place the
cursor inside the main chromatogram.
Tags
AU
SA
FullView Print Del Tag Activity
Edit View Utilities Options
Edit
Method
New
Method
New
Run Manual Setup
Values at Cursor
Current Tagging Trace:
QuadTec-3 (214 nm)
Run Time:
Gradient:
QuadTec-1 (280 nm):
QuadTec-2 (260 nm):
QuadTec-3 (214 nm):
QuadTec-4 (405 nm):
Conductivity:
GP pressure:
No Trace:
00:03:38.4
21% Buffer B
0.0345 AU
0.0228 AU
0.4762 AU
0.0021 AU
8.47 mS/cm
375.53 psi
Protocol
wash
load
1
2
Run Notes Report PostRun Settings Log
Help File
Browser
QuadTec-3 (214 nm) GP pressure
BioLogic Duo-Flow - - - -
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
Split
1.00ml/min 0 %B
Gradient Pump: F-40 UV Conductivity
438 psi 0.03775 AU 359 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.953 Volt 0.00 pH
50
100% Buffer B
AU
50.0
0.0
-10.0
40.0
30.0
20.0
10.0
Fractions A
mS/cm
B
0.00
1F
1
A
2F 3F 4F 5F 6F 7F 8F 9F 10F 11F 12F 13F14F
4.00 2.00 6.00
2
3
4
0.100
0.075
0.050
0.025
-0.000
Min. Tenth
Rack Pos:
Grid #:
MODES OF OPERATION SYSTEM OPERATION
7-39