8 data analysis windows, Quantification tab, Chapter 8. data analysis windows – Bio-Rad Firmware & Software Updates User Manual
Page 98: Data analysis windows
CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
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8
Data Analysis Windows
Read this chapter for more information about the tabs in the Data Analysis window:
•
Quantification tab (page 85)
•
Quantification Data tab (page 89)
•
Melt Curve tab (page 92)
•
Melt Curve Data tab (page 93)
•
End Point tab (page 96)
•
Allelic Discrimination tab (page 98)
•
Custom Data View tab (page 100)
•
QC tab (page 101)
•
Run Information tab (page 102)
•
Data file reports (page 103)
•
Well Groups Reports (page 106)
NOTE: The tabs displayed in the data analysis window can be customized by
selecting them in the View menu. This layout will be saved with the file.
Quantification Tab
Use the data in the Quantification tab (Figure 60) to set the data analysis conditions, including
the baseline settings for individual wells and the threshold settings. The Quantification tab
shows data in these four views:
•
Amplification chart. Shows the relative fluorescence units (RFU) for each well at every
cycle. Each trace in the chart represents data from a single fluorophore in one well
•
Standard curve. This graph is shown only if the run includes wells designated as
Sample Type Standard. It shows a standard curve with the threshold cycle plotted
against the log of the starting quantity. The legend shows the Reaction Efficiency (E) for
each fluorophore in the wells with a standard sample type
•
Well selector. Selects the wells with the fluorescence data you want to show
•
Spreadsheet. Shows a spreadsheet of the data collected in the selected wells