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3 performing runs, Run setup window, Chapter 3. performing runs – Bio-Rad Firmware & Software Updates User Manual

Page 40: Performing runs

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CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual

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3

Performing Runs

Read this chapter for information about performing runs using CFX Manager™ software:

Run Setup window (page 27)

PrimePCR™ runs (page 28)

Protocol tab (page 29)

Plate tab (page 29)

Start Run tab (page 30)

Run Details window (page 31)

Instrument Summary window (page 34)

Run Setup Window

The Run Setup window provides quick access to the files and settings needed to set up and
start a run. To open the Run Setup window, perform one of these options:

Click the User-defined or the PrimePCR button in the Run Setup tab of the Startup
Wizard (page 17)

Click the User-defined Run Setup or PrimePCR Run Setup button in the main
software toolbar (page 16)

Select File > New > User-defined Run or PrimePCR Run in the main software
menu bar (page 14)

The Run Setup window includes three tabs:

Protocol. Click the Protocol tab to select an existing protocol to run or edit, or to create
a new protocol in the Protocol Editor window (page 37)

Plate. Click the Plate tab to select an existing plate to run or edit, or to create a new
plate in the Plate Editor window (page 45)

Start Run. Click the Start Run tab (page 30) to check the run settings, select one or
more instrument blocks, and begin the run

NOTE: If the protocol currently selected in the Protocol tab does not include a step
with a plate read for real-time PCR analysis, then the Plate tab is hidden. To view
the Plate tab, add a “Plate Read” (page 39) in at least one step in the protocol.

NOTE: Start a new run from a previous run by selecting File > Repeat a Run in the
main software menu bar or Repeat Run in the Startup Wizard. Select the data file
(.pcrd) for the run you want to repeat.