Bio-Rad Firmware & Software Updates - Manual (Page 75)

Stand-Alone Operation
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include the temperature, time, gradient, ramp rate, increment, extension, and add/
remove a plate read (Figure 40).
Figure 40. Step Options window.
NOTE: A gradient ranging from 1–24 o C can be entered. Once a step has a gradient,
you can edit the gradient temperature by selecting the upper and lower
temperature buttons in the graphic view without opening the Step Options screen.
5. The GOTO step instructs the thermal cycler to repeat a set of steps in a loop to create
the cycles in the PCR run. Select a GOTO step and touch the appropriate button to
change the step or the number of repeats.
Changing Run Parameters
• To change the sample volume, touch the Volume field (Figure 39). Enter a new
sample volume in microliters. The sample volume you enter determines the
temperature control mode that is used during a run.
TIP: Entering a sample volume from 1 to 50 for CFX96 Touch system, 1 to 125 for
CFX96 Touch Deep Well system, or 1 to 30 for CFX384 Touch system, selects
Temperature Control mode, which is the standard mode. Entering zero (0) selects
Block mode. Temperature mode is the recommended mode because it most
accurately represents the actual sample temperature.
• To change the lid temperature, touch the Lid field and enter a new temperature
(Figure 39). The default lid temperatures for the CFX96 and CFX384 modules are
105
o C and 95 o C, respectively.
Saving a Protocol
• Once a protocol has been created, you have the option to save it. Touching the Save
button will display the Save As window in which the Name can be entered, and the
Location and Folder specified (Figure 41). Touch OK to save.