Bio-Rad Firmware & Software Updates - Manual (Page 166)

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
153
10.When CFX Manager software completes the calibration run, a dialog box appears. Click
Yes to finish calibration and open the Dye Calibration Viewer.
11.Click OK to close the window.
Instrument Maintenance
Your CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch system
includes a sensitive optical shuttle system that moves quickly during data collection and a
sample block that must heat and cool very fast. Contamination of these components can
interfere with thermal cycling and data collection.
WARNING! Never allow a reaction to run with an open or leaking sample lid. The
reagents could escape and coat the block, inner lid, and optical head in the shuttle
system. Excessive dirt can dim the signal, and fluorescence contamination can
create excessive background signal. The shuttle system cannot be cleaned except
by trained Bio-Rad service engineers.
Avoid contaminating the CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384
Touch systems by following these suggestions:
• Always clean the outside of any containers before placing them in the block
• Never run a reaction with a seal that is open, loose, punctured, or otherwise
damaged because you could contaminate the block, inner lid, and optical system
• Never run a PCR or real-time PCR reaction with volatile reagents that could explode
and contaminate the block, inner lid, and optical system
• Clean the block and inner lid periodically to prevent the buildup of dirt, biohazardous
material, or fluorescent solutions (see Cleaning the Optical Reaction Module on
page 153)
• Never clean or otherwise touch the optical system behind the heater plate holes in
the inner lid (Figure 105)
• Clean the outer lid and C1000 Touch™ base or CFX Connect base on a regular
schedule (for details see C1000 thermal cycler instruction manual)
Cleaning the Optical Reaction Module
The block of the optical reaction module should be cleaned, along with the C1000 or CFX
Connect thermal cycler base, on a regular schedule to remove any debris or dirt that might
interfere with proper function. Clean as soon as you discover debris and spilled liquids with a
soft, lint-free cloth that is dampened with water. Cleaning the instrument allows precise
instrument function. For more detailed information about cleaning the C1000 Touch base, see
the C1000 thermal cycler instruction manual.
WARNING! Never use cleaning solutions that are corrosive to aluminum. Avoid
scratching the surface of the C1000 or CFX Connect reaction module bay.
Scratches and damage to this surface interfere with precise thermal control.
WARNING! Never pour water or other solutions into the C1000 or CFX Connect
reaction module bay. Wet components can cause electrical shock when the
thermal cycler is plugged in.
Clean the CFX96, CFX96 Deep Well, CFX Connect, or CFX384 optical reaction module as
soon as you discover debris, dirt, or contamination in the block or on the inner lid. Any dirt can
interfere with the ability of the block to change temperature quickly and collect accurate
fluorescence data. To clean the reaction module, follow these guidelines.