Bio-Rad Firmware & Software Updates - Manual (Page 161)

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Creating a LIMS Protocol
To start a LIMS run, a CFX Manager software protocol file (*.prcl) must be created and saved in
the designated LIMS protocol folder location specified in the LIMS tab of the Options window.
Creating a LIMS File
A LIMS file (*.plrn) contains the plate setup details and the protocol file name. This file is
generated by your internal LIMS. CFX Manager software will use the LIMS file to create a plate
file that will be used in conjunction with the named protocol file to start a run and generate
data.
The following steps should be performed by a LIMS specialist.
1. Select View > Show > LIMS File Folder from the main software menu bar.
2. Select either CFX96 LIMS Plate Import Template.csv or CFX384 LIMS Plate Import
Template.csv and import into your internal LIMS (Figure 102).
Figure 102. Opening the LIMS CFX96 Plate Import Template.
3. Using the LIMS, complete the template by filling in the required fields as listed in
Table 45.
4. Save the template with the file name extension .plrn directly to the designated LIMS file
folder location.
WARNING! Changing the file extension from .csv to .plrn is required for CFX
Manager software to recognize the file and start a LIMS run.
Table 45. Definition of LIMS .csv file contents.
Column Row Description Content Purpose
A 1 Plate Header Do not edit Predefined
A,B,C 2 Field/Data/
Instruction Do not edit Predefined
B 3 Version Do not edit Predefined
B 4 Plate Size Do not edit Predefined
B 5 Plate Type Enter “BR White,” “BR Clear,” or other
calibrated plate type Required