Bio-Rad Firmware & Software Updates - Manual (Page 41)

Performing Runs
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The Run Setup window opens with the Protocol tab in front (Figure 20). To open another tab,
click that tab or click the Prev or Next button at the bottom of the window.
Figure 20. Run Setup window, including the Protocol, Plate, and Start Run tabs.
PrimePCR Runs
PrimePCR runs use pathway or disease-specific assays that have been wet-lab validated and
optimized and are available from Bio-Rad in the following formats:
• Pre-plated panels. Plates contain assays that are specific for a biological pathway
or disease
• Custom configured plates. Plates can be set up in a user-defined layout with the
option to choose assays for targets of interest, controls, and references
• Individual assays. Tubes contain individual primer sets for use in real-time reactions
Select one of the following options to start a PrimePCR run:
• PrimePCR from the Run setup tab on the Startup Wizard
• A PrimePCR run from the Recent Runs list of the Repeat run tab on the Startup
Wizard
• File > New > PrimePCR run from the main window
• File > Open > PrimePCR Run File... from the main window
• Drag and drop a PrimePCR run file onto the main window of CFX Manager
Once a PrimePCR run has been selected, the Run Setup window will open on the Start Run
tab with the default PrimePCR protocol and plate layout loaded based on the instrument type
selected.
To reduce the overall run time, the melt step can be removed by unchecking the box adjacent
to Include Melt Step on the Protocol tab. Any other modifications to a PrimePCR run
protocol are not recommended. The default protocol was used for assay validation. Any
deviation from this may affect the results. Protocol changes will be noted in the Run
Information tab of the resultant data file and in any reports that are created.