Bio-Rad Firmware & Software Updates - Manual (Page 112)

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
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• Well spreadsheet. Shows a spreadsheet listing the allelic discrimination data collected
in the selected wells
• Setting Panel. Allows changes to x and y axis label in the Allelic Discrimination chart,
selection of the cycle for analysis, and selection to display call map
Figure 74. Layout of the Allelic Discrimination tab in the Data Analysis window.
Adjusting Data for Allelic Discrimination
The software automatically assigns a genotype to wells with unknown samples based on the
positions of the NTCs and the angle and distance of the unknown data points from the NTCs.
Adjust allelic discrimination data by using any of these methods:
• Select a fluorophore for each axis in the chart (X: and Y:) in the settings panel in the
bottom right of the window
• Change a call manually by left-click-and-drag to select the data point(s) in the Allelic
Discrimination chart and then selecting an option in the Selected Wells (including
Allele 1, Allele 2, Heterozygote, No Call, Undetermined, Auto Call). You may select
Auto Call to revert to default call by the software.