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Results spreadsheet, Standard curve results spreadsheet – Bio-Rad Firmware & Software Updates User Manual

Page 103

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Data Analysis Windows

90

Results Spreadsheet

Select a Results spreadsheet (Figure 64) to see data for each well in the plate.

Figure 64. Quantification Data tab with Results spreadsheet selected.

NOTE: All Std. Dev (standard deviation) calculations apply to the replicate groups
assigned in the wells in the Plate Editor window. The calculations average the C

q

value for each well in the replicate group.

The Results spreadsheet includes the types of information listed in Table 26.

Standard Curve Results Spreadsheet

Select the Standard Curve Results spreadsheet (Figure 65) to see the calculated standard
curve parameters.

Table 26. Results spreadsheet content.

Information

Description

Well

Well position in the plate

Fluor

Fluorophore detected

Target

Amplification target name (gene)

Content

Sample type and Replicate #

Sample

Sample description

Biological Set Name

Name of the biological set

C

q

Quantification cycle

C

q

Mean

Mean of the quantification cycle for the replicate group

C

q

Std. Dev

Standard deviation of the quantification cycle for the replicate
group

Starting Quantity (SQ)

Estimate of the starting quantity of the target

Log Starting Quantity

Log of the starting quantity

SQ Mean

Mean of the starting quantity

SQ Std. Dev

Standard deviation of the starting quantity across replicates

Set Point

Temperature of sample in the well for a gradient step across
replicates