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Bio-Rad PCR Plastic Accessories User Manual

Microseal, F’ sealing foil, For pcr plates

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Product

Product Highlights

Compatibility

Microseal ‘F’ foil

MSF-1001

(100 sheets)

• Foil can be pierced for sample recovery
• Plate storage (ambient to –70°C)
• PCR volumes ≥25 µl (96-well) or ≥5 µl (384-well)

All PCR plates

Strip caps

TCS-series

• Tight sealing for thermal cycling, storage, or transport
• Choice of dome caps or ultraclear, flat optical caps
• PCR volumes ≥5 µl

96-well polypropylene

PCR plates and

0.2 ml tubes

Microseal ‘A’ film

MSA-5001

(50 sheets)

• Smooth application and release — no aerosol generation
• Peel-away release liner for pristine sealing surface
• PCR volumes ≥10 µl (96-well) or ≥5 µl (384-well)

All PCR plates,

tube strips

Microseal ‘B’

adhesive seals

MSB-1001

(100 sheets)

• Clear adhesive seal for thermal cycling or fluorescent assays
• Plate storage or transport before or after cycling

(ambient to –40°C)

• PCR volumes ≥10 µl (96-well) or ≥5 µl (384-well)

All polypropylene

PCR plates

96-well PCR plate

sealing mats

223-9442

(5 mats)

• Reusable mat — ideal for sequencing
• Smooth release — no aerosol generation
• PCR volumes ≥10 µl

96-well polypropylene

PCR plates

Microseal ‘P’ and

‘P+’ Pads

MSP-1001

MSP-1002

(10 pads)

• Reusable sealing pad adheres to the heated lid
• Use MSP-1001 with Power Bonnet

motorized lid

(ALP-1296, ALP-1238), using PCR volumes ≥15 µl (96-well)

or ≥10 µl (384-well)

• Use MSP-1002 with Moto Alpha

motorized lid

(ALP-2296, ALP-2238), using PCR volumes ≥10 µl (96-well)

or ≥5 µl (384-well)

All 384-well and

96-well PCR plates

with raised well rims

Microseal

®

‘F’ Sealing Foil

for PCR Plates

This aluminized foil serves as an exceptional barrier against evaporation from –70°C to
105°C. It is thin enough to pierce with a pipet tip to recover samples from individual wells.
Minimum recommended PCR sample volumes are 25 µl in 96-well PCR plates and 5 µl in
384-well plates.

Directions for Use
1. Remove white liner and place adhesive surface over wells.
2. Apply pressure with fingers or soft rubber roller to complete contact with each well.
3. Close the heated lid and apply pressure. When using MJ thermal cyclers with adjustable

heated lids, turn the thumbwheel until the lid contacts the plate — when an increase in
resistance is first felt. Continue to turn the thumbwheel about 1/2–3/4 turn past the point
of first contact (1/4 of the thumbwheel circumference is visible, so two passes of the visible
portion equals 1/2 turn).

4. If evaporation occurs at the edges, tighten lid an additional 1/4 turn of the thumbwheel.

Microseal ‘F’ Foil

MSF-1001 100 sheets per package

Related Product

MSR-0001 Sealing roller

Quickly and uniformly presses seal over PCR plate wells

Sealer Comparison Chart