beautypg.com

Right-click menu options, Data spreadsheet – Bio-Rad Firmware & Software Updates User Manual

Page 127

background image

Gene Expression Analysis

114

Coefficient of variation (CV) of normalized reference gene relative quantities. A
lower CV value denotes higher stability

M-value. A measure of the reference gene expression stability

Right-Click Menu Options

Right-click on the bar chart to select the items shown in Table 37.

Data Spreadsheet

Table 38 describes the information shown in the bar chart spreadsheet.

Table 36. Acceptable values for stably expressed reference genes.
(Hellemans et al. 2007)

Samples

CV

M

Homogeneous

<0.25

<0.5

Heterogeneous

<0.5

<1

Table 37. Right-click menu items.

Item

Function

Copy

Copy the chart to a clipboard

Save Image As...

Save the chart as an image file. Set the resolution and
dimensions of the image and then select the file type (PNG,
GIF, JPG, TIF, or BMP)

Page Setup...

Select a page setup for printing

Print...

Print the chart

Set Scale to Default

Select Show All to have all of the data visible in the bar chart
frame or Scroll Bar to have a scroll bar appear if there are too
many samples to display in the chart frame while maintaining a
minimum bar width

Chart Options...

Open the Chart Options window to adjust the graph

Sort...

Sort the order samples or targets appear on the chart x-axis

User Corrected Std Devs

Calculate the error bars using the corrected standard deviation
formula

Use Solid Bar Colors

Display solid bars in the chart

X Axis Labels

Choose to display x-axis labels horizontaly or angled

Table 38. Description of information in the spreadsheet on the Bar Chart tab.

Information

Description

Target

Target Name (amplified gene) selected in the Experiment
Settings window

Sample

Sample Name selected in the Experiment Settings window

Ctrl

Control sample, when the Sample Name is selected as a
control in the Experiment Settings window

Expression

Normalized Gene Expression (

ΔΔC

q

) or Relative Quantity (

ΔC

q

)

depending on the selected mode

Expression SEM (or SD)

Standard error of the mean or standard deviation, depending
on the selected option