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Bio-Rad Firmware & Software Updates User Manual

Page 162

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CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual

149

B

6

Scan Mode

Enter “SYBR/FAM Only:,” “All
Channels,” or “FRET”

Required

B

7

Units

Enter one of the following “copy
number,” “fold dilution,” “micromoles,”
“nanomoles,” “picomoles,”
“femtomoles,” “attomoles,”
“milligrams,” “micrograms,”
“nanograms,” “picograms,”
“femtograms,” “attograms,” or
“percent”

Required

B

8

Run ID

Enter short description or bar code
identifying this run

Optional

B

9

Run Note

Enter run description

Optional

B

10

Run Protocol

Enter protocol file name exactly as listed Required

A

11-15

TBD/Empty

Do not edit

Predefined

A

16

Plate Data

Do not edit

Predefined

A

14-110

Well Position

Do not edit

Predefined

B-G

Ch1 Dye, Ch2
Dye, Ch3 Dye,
Ch4 Dye, Ch5
Dye, FRET

Enter one calibrated dye name (for
example, “FAM”) for each channel being
used

Required

H

Sample Type

Enter one of the following sample types
“Unknown,” “Standard,” “Positive
Control,” “Negative Control,” “NTC,” or
“NRT”

Required

I

Sample Name

Enter sample name

Optional

J-O

CH1 Target,
CH2 Target,
CH3 Target,
CH4 Target,
CH5 Target,
FRET Target,

Enter target name for each channel
used

Optional

P

Biological Set
Name

Enter biological set name

Optional

Q

Replicate

Enter a positive integer for each set of
replicates. The value cannot be zero

Optional

R-W

CH1 Quantity,
CH2 Quantity,
CH3 Quantity,
CH4 Quantity,
CH5 Quantity,
FRET Quantity

Enter quantity values for any standards.
Enter concentration in decimal form

Required for
all standards

X

Well Note

Enter well note

Optional

Table 45. Definition of LIMS .csv file contents.

Column

Row

Description

Content

Purpose