Bio-Rad Firmware & Software Updates - Manual (Page 144)

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
131
Regulation
Regulation is a measure of the increase or decrease in the expression of a target for an
experimental versus a control sample and is determined as follows:
Where:
• RNE = Relative normalized expression; this is the same as normalized expression
when a control is selected
P-Value
The p-value is a measure of the statistical significance of an experimental sample data point
compared to a control.
NOTE: At least two replicates are required for both control and experimental
samples for a p-value to be determined. The more replicates that are present, the
greater the accuracy.
The p-value is calculated as follows:
Where:
•
Where:
• Γ = gamma function
•
t = t-statistic
Where:
• Mean = Arithmetic mean
• NE = Normalized expression
• Count(x) = Size of list x
• SD = Sample standard deviation
For RNE 1 Regulation , ≥ RNE =
For RNE 1 Regulation , < 1 – ( ) RNE ⁄ =
p-value 1 A – =
2
1
2
1
2
2
1
+
−
− =






+






Γ





 +
Γ
= =

v
t
t x
v
x
v
v
v
A
π
v Count (NE
sample (Experimental)
) Count (NE
sample (Control)
) 2 – + =