Bio-Rad Firmware & Software Updates - Manual (Page 164)

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
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When Automatically Export Data after Run is selected in LIMS Options, a second data file
compatible with LIMS data retrieval will be saved to the same location. The file format and
contents are defined using LIMS Data Export Format settings. To export this data manually,
select Export > Export to LIMS Folder from the main software window menu bar.
Calibration Wizard
The CFX96 Touch, CFX96 Touch Deep Well, and CFX Connect systems are factory calibrated
for commonly used fluorophores in white-well and clear-well plates. The CFX384 Touch
system is factory calibrated for the same fluorophores in white-well plates only (Table 46).
The CFX96 Touch system, CFX96 Touch Deep Well system, CFX Connect system, and the
CFX384 Touch system also includes a channel dedicated to FRET chemistry; this channel
does not require calibration for specific dyes.
To open the Calibration Wizard to calibrate the CFX96 Touch, CFX96 Touch Deep Well, CFX
Connect, or CFX384 Touch real-time PCR system:
1. Select an instrument in the detected instruments pane.
2. Select Tool > Calibration Wizard to open the window and calibrate new dye and plate
combinations (Figure 104).
Table 46. Factory calibrated fluorophores, channels, and instruments.
Fluorophores Channel
Excitation
(nm) Detection
(nm)
Instrument
FAM, SYBR
®
Green I
1 450-490 515-530 CFX96 Touch, CFX96 Touch
Deep Well, CFX Connect, and
CFX384 Touch systems
VIC, HEX, CAL Fluor
Gold 540, Cal Fluor
Orange 560 2 515-535 560-580 CFX96 Touch, CFX96 Touch
Deep Well, CFX Connect, and
CFX384 Touch systems
ROX, Texas Red, CAL
Fluor Red 610, TEX 615 3 560-590 610-650 CFX96 Touch, CFX96 Touch
Deep Well, and CFX384 Touch
systems
CY5, Quasar 670 4 620-650 675-690 CFX96 Touch, CFX96 Touch
Deep Well, and CFX384 Touch
systems
Quasar 705, Cy5.5 5 672-684 705-730 CFX96 Touch and CFX96
Touch Deep Well systems only