Bio-Rad iTaq™ DNA Polymerase - Manual

iTaq ™ DNA Polymerase
250 U (50 µl) 170-8870
5,000 U (1 ml) 170-8875
For research purposes only
Store at -20°C
Storage and Stability
Store the iTaq DNA Polymerase at -20°C in a constant temperature freezer. When stored
under these conditions the polymerase is stable for one year after ship date.
Kit Contents
iTaq DNA Polymerase is suitable for many PCR applications. the antibody-mediated hot-start
employed by iTaq Polymerase sequesters Taq activity prior to the initial PCR denaturation
step. Upon heat activation for three minutes at 95°C, the antibodies denature irreversibly,
releasing fully active Taq DNA polymerase. This enables specific and efficient primer
extension with the convenience of room temperature reaction assembly.
Reagent Kit Size Volume Description
iTaq DNA polymerase 250 U 50 µl iTaq DNA polymerase, 5 U/µl
(Clear Cap)
10X iTaq Buffer
1.25 ml 10X PCR buffer, 200 mM Tris-HCl,
(Blue Cap) pH 8.4, 500 mM KCl
MgCl
2
1.25 ml 50 mM MgCl
2
(Green Cap)
iTaq DNA polymerase 5,000 U 1 ml iTaq DNA polymerase, 5 U/µl
(Clear Cap)
10X iTaq Buffer
25 ml 10X PCR buffer, 200 mM Tris-HCl,
(Bottle) pH 8.4, 500 mM KCl
MgCl
2
25 ml 50 mM MgCl
2
(Bottle)
Reaction Set Up
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10X iTaq buffer 5.0 µl 1X
50 mM MgCI
2
1.5 µl 1.5 mM
10 mM dNTP mix 1.0 µl 200 µM each
iTaq DNA Polymerase 0.25 µl 1.25 U
Primer 1 x µl 100 nM–500 nM
Primer 2 x µl 100 nM–500 nM
Sterile water x µl
DNA template x µl
Total Volume 50 µl
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