Bio-Rad Firmware & Software Updates - Manual (Page 76)

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
63
Figure 41. Save As window.
Running the Protocol
1. To run the protocol, touch the Run button in the Edit Protocol window (Figure 39).
2. Verify the lid temperature (Lid Temp), sample volume (Volume), and file name (File Name
(.zpcr)) that will be used for the run (Figure 42). A default stand-alone data file name is
created prior to the run.
Figure 42. Run confirmation screen.
3. Select a Scan Mode to specify the channels in which fluorescence data should be
collected during a run. Scan modes detect calibrated fluorophores in these channels:
• SYBR/FAM. Collects data only from channel 1 and provides a fast scan
• All Channels. Collects data from channels 1 through 5 on the CFX96 Touch and
CFX96 Touch Deep Well systems, or channels 1 through 4 on the CFX384 Touch
system
• FRET. Collects data only from the FRET channel and provides a fast scan
4. (Optional) The Additional Settings window permits the entry of User Name, Email
Address, Sample ID, Ramp Rate, and presents the option to run with the ramp rate of a
DNA Engine thermal cycler.
5. Touch OK to start the run.