Bio-Rad Firmware & Software Updates - Manual (Page 113)

Data Analysis Windows
100
Chart Menu Options
In addition to the common right-click menu options to copy, print, and export charts (see Table
22), Table 33 lists the menu options available on the Allelic Discrimination chart.
Allelic Discrimination Spreadsheet
The Allelic Discrimination spreadsheet at the top right side of the Allelic Discrimination tab
displays the information shown in Table 34.
Custom Data View Tab
The Custom Data View tab simultaneously displays multiple panes in a customizable format
(Figure 75).
The Load a Preset View drop-down list offers a selection of display format templates. The
default view displayed is dependent on the file being analyzed. For example, if Melt Curve data
are present, the Amp+Melt default view is displayed.
The data view can be further customized by:
• Selecting an alternate preset view from the drop-down list
• Using the drop-down menu located at the top of an individual pane
• Using the Rows and Columns drop-down selection options
• Changing individual pane dimensions by clicking and dragging the bars at the
periphery of each pane
Table 33. Allelic Discrmination chart right-click (and drag) and left-click (and drag) menu
options.
Menu Option Function
Zoom Focus into selected area when more than one data point is
selected
Well XX Change call, view only this well, remove this well from view,
set color for this trace, or exclude this well from analysis
when clicked on a single trace
Selected Wells Change calls, view only these wells, remove these wells
from view, set color for these traces, or exclude these wells
from analysis when multiple traces are selected
Table 34. Allelic Discrimination spreadsheet contents.
Information Description
Well Well position in the plate
Sample Sample name/description
Call Identity of the allele, including automatic Allele 1, Allele 2,
Heterozygote, No Call, or Undetermined
RFU1 RFU for Allele1
RFU2 RFU for Allele2
Type Auto (Automatic) or Manual. Describes the way the call was
made. Automatic means the software selected the call.
Manual means the call was chosen by the user