Bio-Rad Firmware & Software Updates - Manual (Page 35)

CFX Manager™ Software
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Master Mix Calculator
To open the Master Mix Calculator, click the Master Mix Calculator button in the toolbar
(Table 9) or select Tools > Master Mix Calculator from the main window.
Figure 15. Master Mix Calculator window.
To set up a reaction master mix:
1. Select either SYBR
®
Green/EvaGreen or Probes detection method.
2. Edit the default target name by highlighting the target name in the drop-down target list,
entering a new target name in the Target box, and pressing Enter on the keyboard.
3. Enter the starting and final concentrations for your forward and reverse primers and any
probes.
4. Additional targets can be added by clicking the New button. To delete targets, select the
target using the drop-down target list and click Remove.