Ocean Optics OOISensors - Manual (Page 61)

3: Oxygen Sensors
2. Set the integration time for the entire calibration procedure when the probe is measuring the
standard with zero concentration at the lowest sample temperature you will be using. The
fluorescence peak (~600 nm) will be at its maximum at zero concentration.
3. Adjust the integration time so that the fluorescence peak does not exceed 3500 counts. Set the
integration time to powers of two (2, 4, 8, 16, 32, 64 etc.) to ensure a constant number of LED
pulses during the integration time. (The intensity of the LED peak [~475 nm] does not affect your
measurements.)
4. Click on the Dark Measurement icon to take a Dark measurement.
5. Select Calibrate | Oxygen, Multiple Temperature from the menu bar. The Multiple
Temperature Calibration screen appears.
6. Enter the serial number of the probe in the S/N box. The Date box should display the current
date. The file name and path will appear under Calibration File Path once you select File | Save
Calibration Chart and save the chart. At the bottom of the screen is a box to assign a text label
to the Oxygen Concentration Units.
Calibrating Standards without Using Factory Calibration – Part 1
► Procedure
1. Enter the concentration values for each standard with known oxygen concentrations in the top
row of the table. The first standard should have 0% oxygen concentration, such as in a nitrogen
flow or in a solution of sodium hydrosulfite
2. Leave the oxygen probe in the standard for at least 5 minutes. This guarantees equilibrium.
3. Manually enter the temperature of this standard in the second cell of the left column or select
Spectrometer | Sample Temperature from the menu.
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