Ocean Optics OOISensors - Manual (Page 54)

3: Oxygen Sensors
Calibrating Oxygen Sensors
The following sections provide instructions on calibrating your oxygen sensors both with and without
temperature compensation.
Calibrating Oxygen Sensors without Temperature
Compensation
Perform the steps below to calibrate your oxygen sensors without using temperature compensation:
Preparing for Calibration
► Procedure
1. Set the data acquisition parameters for the calibration procedure (integration time, averaging, and
boxcar smoothing).
2. Set the integration time for the entire calibration procedure when the probe is measuring the
standard with zero concentration. The fluorescence peak (~600 nm) will be at its maximum at
zero concentration.
3. Adjust the integration time so that the fluorescence peak does not exceed 3500 counts.
Note
If you are using the LS-450 in Pulse mode, set the integration time to powers of 2 (2, 4, 8, 16, 32,
64 etc.) to ensure a constant number of LED pulses during the integration time.
• The intensity of the LED peak (~475 nm) does not affect your measurements.
4. Take a Dark measurement.
5. Select Calibrate | Oxygen, Single Temperature from the menu bar. The Single Temperature
Calibration screen appears.
6. Enter the serial number of the probe in the S/N box. The Date box should display the current
date. The file name and path will appear under Calibration File Path once you select File | Save
Calibration Chart and save the chart. At the bottom of the screen is a box to assign a text label
to the Oxygen Concentration Units.
7. Select Multi Point from the drop-down menu located next to Calibration Type.
8. Locate the Channel section and select the spectrometer channel associated with the sensor you
are calibrating.
44 FOXY-AL300-000-02-0207