Ocean Optics OOISensors - Manual (Page 56)

3: Oxygen Sensors
5. Once you click the green Scan Standard button, a red Scanning button appears. Watch the
values in the Intensity column. When there appears to be no changes in this value, select the red
Scanning button to accept the intensity value.
Note
If the probe has an overcoat, then the standards used in the calibration can be of a state of matter
different from the sample that you are using. You could switch between gas and liquid and your
calibration data will be valid. If the probe does not have an overcoat, standards used in the
calibration not only must be of the same state of matter as your sample, but also must have the
same refractive index as your sample.
6. Enter the known oxygen concentration of the standard in the Concentration column and leave
the oxygen probe in the standard for 5 minutes.
7. Move the cursor to the Intensity column.
8. Click the green Scan Standard button or select Spectrometer | Scan Standard from the menu
bar. The intensity of the standard should then appear in the Intensity column.
9. Repeat the steps above for each standard you wish to measure.
You have now calibrated all standards. Proceed to Finalizing Calibration .
Finalizing Calibration
► Procedure
1. Click the green Curve Fit button. A graph displaying the results of your calibration procedure
appears in the bottom of the screen. Verify the results of the calibration, and then proceed to Step
2.
2. Click the green Update Channel Calibration button to save information from this calibration
procedure for the specified spectrometer channel into data files.
3. Save the Calibration Table for future use. Select File | Save Calibration Table from the menu
bar.
4. To print the entire screen, select File | Print from the menu bar.
5. Select File | Close from the menu bar to return to the main display window.
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