Ocean Optics OOISensors - Manual (Page 48)

3: Oxygen Sensors
Fluorescence Quenching
The following paragraph explains the principle of fluorescence quenching as it relates to oxygen sensors.
Oxygen, as a triplet molecule, is able to quench efficiently the fluorescence and phosphorescence of
certain luminophores. This effect (first described by Kautsky in 1939) is called "dynamic fluorescence
quenching." Collision of an oxygen molecule with a fluorophore (in the case of oxygen sensors, a
ruthenium complex) in its excited state leads to a non-radiative transfer of energy. The degree of
fluorescence quenching relates to the frequency of collisions, and therefore to the concentration, pressure
and temperature of the oxygen-containing media.
Connecting the Fiber Optic Sensors System
Once you have configured system hardware and installed OOISensors, you will need to connect the
sampling optics (probes, optical fiber assemblies) to the Fiber Optic Sensors System.
► Procedure
Follow the steps below to properly connect the sampling optics:
1. Connect the probe to the bifurcated optical fiber assembly that came with the Fiber Optic Sensors
System. To obtain operating instructions for specific probes, consult the Ocean Optics website or
the documentation that accompanied the probe.
2. Locate the 21-02 SMA Splice Bushing that came with the system. This item is a 0.75" screw with
two female ends.
3. Screw one end of the splice bushing into the SMA 905 connector on the proximal end of the
probe.
4. Locate the bifurcated fiber that came with the system. This optical fiber assembly has a “Y”
shaped design.
5. Connect the common end of the bifurcated fiber to the splice bushing/probe.
6. Connect one arm of the bifurcated fiber to the SMA connector on the spectrometer, and then
connect the other arm to the SMA connector on the LS-450 Blue LED light source.
Note
It does not matter which arm of the fiber is connected to the light source or spectrometer.
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