Ocean Optics OOISensors User Manual
Page 120
7: Troubleshooting
Issue Possible
Cause Resolution
Peaks displayed in
the graph screen are
in incorrect places.
Wavelength coefficients may
be incorrect, or probe overcoat
may not be in place or may be
damaged.
Reload or re-enter the wavelength coefficients
correctly.
Confirm that the probe overcoat is in place.
Incorrect counts
obtained on
Calibration screen
Incorrect fluorescence peak
entered or dark measurement
taken incorrectly.
Ensure that the fluorescence peak is entered
correctly on the Configuration | Spectrometer
screen. Verify that the LED was not on while the
dark measurement was obtained, and retake the
dark measurement if required.
Note:
If Enable Reference is selected on the
Sensors tab of the Configure Spectrometer
screen, you will not see counts. Rather, you will
see the ratio of the fluorescence peak to the
LED peak.
Large peak at 900
nm with little
fluorescence signal
There is a broken fiber inside
probe and/or loose splice
bushings.
Verify the condition of the fiber inside the probe
and replace the probe if necessary. Tighten all
splice bushings to ensure maximum
performance.
Signal decay is
excessively quick
There may be solvents or
harmful chemicals in the
sample, or you may have
autoclaved or washed the
probe with solvents.
Remove any solvents or harmful chemicals from
the sample you are measuring. Furthermore,
you should never autoclave or wash the probe
with solvents. For information on chemicals
harmful to Ocean Optics probes, see
Effects on Oxygen Probes
.
Unstable readings
A variety of possible causes,
including loose fittings, low
averaging, and probes or
coupling fibers moving during
measurements.
Double-check and tighten all fittings.
Increase signal averaging to at least 20.
Immobilize probe during sample measurements.
If these suggestions do not correct the unstable
readings, contact Ocean Optics technical
support for assistance.
Calibration settings
not retained
You may have disconnected
the probe between sample
measurements. Additionally,
overcoat condition and sample
temperature can affect
calibration.
Ensure that the probe overcoat is intact when
moving the probe from gas to liquids.
Confirm that the sample temperature is constant
when taking sample measurements.
Keep the probe connected to the spectrometer
in between sample measurements.
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