Ocean Optics OOISensors - Manual (Page 58)

3: Oxygen Sensors
3. Adjust the integration time so that the fluorescence peak does not exceed 3500 counts. Set the
integration time to powers of two (2, 4, 8, 16, 32, 64 etc.) to ensure a constant number of LED
pulses during the integration time. (The intensity of the LED peak [~475 nm] does not affect your
measurements.)
4. Select Calibrate | Oxygen, Multiple Temperature from the menu bar. The Multiple
Temperature Calibration screen appears.
5. Select File | Open Calibration Table from the menu bar. The name of the file corresponds to the
serial number of the probe that you are calibrating. Once this file is opened, the Calibration Table
should be populated with oxygen concentration amounts and temperatures.
Note
The serial number is etched on the SMA connector, located at the top of the probe.
6. Select the green Curve Fit button. Graphs displaying the curves appear in the bottom of the
dialog box. Verify that the graphs are satisfactory, and then proceed to Step 7.
7. Click the green Update Channel Calibration button to save information from this calibration
procedure.
8. Select File | Close from the menu bar to return to the main display window.
You have now configured the system for calibration using the factory calibration file. Proceed to the next
section to calibrate your standards.
Note
Second order is strongly recommended for all temperature compensation.
Calibrating Standards Using Factory Calibration
► Procedure
1. Select Calibrate | Oxygen, Single Temperature from the OOISensors menu bar.
2. Click on the Calibration Type drop-down menu and select Single Point.
3. Click on the Curve Fitting drop-down menu and select Second Order Polynomial.
4. Enter the known oxygen concentration of your standard under Concentration.
5. Change the switch in the Temp Compensation section of the Single Temperature Calibration
screen to Yes.
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