Bio-Rad ChromLab™ Software - Manual (Page 97)

Connecting the Sample Injection Loops
Instrument Guide | 95
Connecting the Sample Injection Loops
The NGC chromatography systems support a variety of injection loop types and
sizes, including static loops and dynamic loops. Table 6 on page 77 lists the
supported loops.
Note: You might have already connected a loop if you completed all of the
steps in the section Plumbing and Priming the NGC Chromatography Systems
in the NGC Chromatography Systems and ChromLab Software Installation
Guide.
To connect a loop to the sample inject valve
 Connect one end of the loop to Loop E and the other end to Loop F on the
sample inject valve.
During Load, sample enters through Loop F while air, buffer, or excess sample is
pushed out through Loop E to waste.
During Injection
, buffer enters through Loop E and pushes the sample out to the
column through Loop F.